The Indiana Biobank can receive many types of biological specimens. Current specimen types banked at Indiana Biobank are listed below. If the specimen type needed for your project is not listed, please contact us to see if we can add it. We are always eager to expand our collections.
Standard Collection Tubes
Conical tubes are utilized as the collection receptacle for the CSF upon extraction. 50.0 ml conical tubes are commonly used by Indiana Biobank for this purpose. Lumbar puncture trays may also be provided for the collection of CSF. These trays may include: introducer needles, plastic leur lock syringes, and lidocaine.
Standard Processing Procedures
After CSF collection, the entire specimen is inverted to mix specimen and centrifuged within 15 minutes.
CSF is aliquoted into pre-chilled polypropylene 2.0 ml tubes according to the study protocol.
Standard Collection Tubes
Whole Blood & Buffy Coat
The EDTA (Ethylene Diamine Tetra-acetic Acid) tube is utilized in clinical settings to collect whole blood for the purpose of extracting plasma and buffy coat. The EDTA tube in the kit may be plastic or glass.
Saliva
The Oragene-Discover Saliva Kit is the only kit currently used by Indiana Biobank for the purpose of collecting human saliva for nucleic acid extraction.
Transferred DNA
DNA transferred to Indiana Biobank arrives in the required 2.0 ml screw top polypropylene microcentrifuge tubes: DOT Scientific 819-S 2.0 ml screw cap microcentrifuge tubes with O-rings
Standard Processing Procedures
Whole Blood
EDTA tube is inverted 8-10 times to thoroughly mix additive and specimen prior to centrifugation.
Saliva
Saliva kits are processed at Indiana Biobank. Specimen collection is per industry released instructions.
Standard Collection Tubes
The sodium heparin tube is utilized in clinical settings to collect whole blood for the purpose of extracting PBMCs.
Standard Processing Procedures
The sodium heparin tube is never processed at the collection site.
The tube is inverted 8-10 times to thoroughly mix additive and specimen immediately after blood collection.
Friday or Saturday specimen collection are prohibited for sodium heparin tubes.
Standard Collection Tubes
The EDTA (Ethylene Diamine Tetra-acetic Acid) tube is utilized in clinical settings to collect whole blood for the purpose of extracting plasma and buffy coat. The EDTA tubes may be plastic or glass.
Standard Processing Procedures
The EDTA tube is inverted 8-10 times to thoroughly mix additive and specimen prior to centrifugation.
Specimens will be centrifuged within 30 minutes of specimen collection for the speed and duration dictated by the study protocol. Upon completion of centrifugation, plasma specimens are aliquoted for storage and shipment in polypropylene cryovials.
Standard Collection Tubes
The PAXgene™ tube is utilized in clinical settings to collect whole blood for the purpose of extracting RNA.
Standard Processing Procedures
The PAXgene™ tube is never processed at the collection site
The tube is inverted 8-10 times to thoroughly mix additive and specimen immediately after blood collection.
If freezing prior to shipment, styrofoam rack use is prohibited. Tube racks made of plastic or wire are approved.
Standard Collection Tubes
The Serum or Serum Separator (SST) tubes are utilized in clinical settings to collect whole blood for the purpose of extracting serum.
Standard Processing Procedures
After blood collection, Indiana Biobank's standard procedures dictate whole blood processing begin within 60 minutes of specimen collection.
Immediately after blood collection, the serum tube is inverted 8-10 times to thoroughly mix additive and specimen.
Blood is then clotted by placing the tube within a tube rack at room temperature for 30 minutes.
Specimens will be centrifuged for the speed and duration dictated by the study protocol.
Upon completion of centrifugation, serum specimens are aliquoted for storage and shipment in polypropylene cryovials.
Standard Collection Tubes
Urine may be collected in any polypropylene specimen collection container. Indiana Biobank recommends a wide-mouth leak-resistant container for easier use by research participants.
An example of the preferred container is the Samco™ 90mL 53mm Specimen Container (part number 021090).
Standard Processing Procedures
The urine is transferred to a 15 ml conical tube prior to centrifugation.
Specimens should be centrifuged within 30 minutes of collection at the speed and duration dictated by the study protocol. Upon completion of centrifugation, urine specimens are transferred to the researchers requested aliquot volume.